Protocol – water and sediment sampling for chemical and ecotoxicology analyses


Personal Protective Equipment (PPE) must be used to avoid direct contact with water because of the chemical contaminants and also the large number of dead animals.

The water can be collected from the surface using a plastic bucket or introducing the vial (preferably made of plastic) straight into the river water. Each vial should be filled up to the brim. Then it must be closed, identified and stored in Styrofoam. The samples must be kept cold (4oC approximately) stored in refrigerators or Styrofoam boxes with ice.

For toxicity, 200ml is enough. For chemical analysis, a second sample from the same location should be collected.



The sediment samples can be collected with plastic shovels and transferred to vials or plastic bags. Ideally 1Kg of sediment per sample location is enough.

Since the material will come mixed as in a wave (i.e. unstructured), we suggest collecting the sediment from the surface, deposited on the river margins. If a boat is available, specific tools for sampling the riverbed (core catcher, cylindrical or rectangular corers) can be used.

For samples from the estuarine region/sea, a large volume (additional 1Kg samples) is requested by researchers from IOUSP.

The samples must be stored in Styrofoam boxes with ice or refrigerator (under 4oC).

Sending of water and sediment samples must be done in Styrofoam boxes with ice.